Vitamin-D Receptor (VDR) Gene Polymorphisms (Apai&Bsmi) In Iraqi Osteoporosis Patients

Abstract

Osteoporosis is a polygenic disorder and has been demonstrated to be associated with ~30 candidate genes, the majority of which have also been implicated in the regulation of bone mineral density (BMD). VitaminD receptor (VDR) mediates Vit-D activity, thus VDR gene polymorphismsmay correlate with different diseases. This study aimed todetermine the distribution of VDR gene (ApaI&BsmI) polymorphismsusing a RFLP in in Iraqi osteoporosis patients. The present results revealed There were no significant differences in ApaI genotype(rs7975232) frequencies between the cases and control. the genotypic frequency of heterozygous C/A with homozygous C/C (OR=0.1365, 95% CI=0.0072 - 2.5895, P= 0.1847). and the genotypic frequency of homozygous variant A/A with the homozygous C/C(OR=0.1127,95% CI= 0.0060 - 2.1070, P=0.1440). There were a significant differences in A allele frequencies in patients as compared to the controls inthe VDR ApaI (rs7975232) polymorphism (OR=0.9252, 95% CI =0.4556 - 1.8784, P=0.8295).Also, there were no significant differences in BsmIgenotype (rs15444410 )  frequencies between the cases and control. the genotypic frequency of heterozygous A/G with homozygous G/G (OR=0.3652, 95% CI=0.0402 - 3.3174, P=  0.3709), and the genotypic frequency of homozygous variant A/A with the homozygous G/G (OR=0.2833, 95% CI= 0.0293 - 2.7444, P=  0.2763). There were a significant difference in A allele frequencies in patients as compared to the controls in the CAT C-262T polymorphism (OR= 0.7463, 95% CI=0.4075 - 1.3669, P=0.3433).