Profile of Cytokines (IL-17A, IL-6 and TNF-α) in Sera of Chronic kidney disease of Iraqi patients.

Abstract

      Chronic kidney disease (CKD) is greatly widespread universally and leading to end-stage renal disease (ESRD), cardiovascular disease (CVD), and early death [1]. In this study, 152 samples were collected, including 94 patients with chronic renal impairment who did not reach the dialysis stage and 58 healthy volunteers who did not have any chronic diseases. The purpose of this study was to test  the impact of various inflammatory factors, including interleukin 17A (IL-17A), interleukin 6 (IL-6) and tumor necrosis factors (TNF-α), which may influence the aggravation of chronic kidney disease.                                                                       

      The Samples were collected from patients diagnosed by a physician with CKD before dialysis. Patient samples consist of (62 males and 32 females) While the healthy samples were composed of (36 males and 22 females) .Patients' age distributed between 18-83 years old .

      The serum level of the immuno-cytokines IL-17A, IL-6 and TNF-α were measured using the ELISA technique. The result showed significant increase in the levels of the three immune cytokines has been observed, due to their pro-inflammatory role in CKD patients compared to healthy subjects with significantly (p<0.0001).