Green Synthesis of Silver Nanoparticles by Enterobacter Aerogenes Bacteria in Combination with Antibiotics Against Multidrug Resistance Streptococcus Mitis Isolated from Oral Cavity of Some Dental Caries Patients in Misan City.

Abstract

The present study aimed at the green, ecofriendly, cost-effective synthesis of silver nanoparticles (AgNPs) by using Enterobacter aerogenes bacterial isolated strains(S1, S2 and S3) from the oral cavity of dental caries patients in the center of Misan province, and also investigates their antibacterial effective combination with some traditional antibiotics against MDR Streptococcus mitis isolated from dental caries patients in the center of Misan  province.  The synthesized   of (AgNPs)  nanoparticles were characterized by process of UV-Visible spectrophotometer Transform Fourier   (FT-IR).  The Scanning Electron Microscopy (SEM) image refers to a spherical shape with the size range of the nanoparticles (AgNPs) synthesized was 47.22 – 105.00 nm. The X-ray diffraction (XRD) patterns revealed the crystalline structure of nanoparticles was AgNPs. Transform Fourier Infrared Spectroscopy (FTIR ) analysis refers to a functional group that is implicated with the reduction of silver ion to AgNPs. All of the clinical bacterial strains isolated from the oral cavity of dental caries patients exhibited resistance against conventional antibiotics. The synergistic effect of antibiotics combined with (AgNPs) mentioned an increasing percentage of antibiotics. However, the maximum of synergistic effect was done only with Erythromycin E 30.7mm for the AgNPs (0.1 mg/ml ), when in contrast Streptomycin 40mm were Streptomycin with the AgNPs 0.1 mg ̷ ml recorded 6.0mm, were Tetracycline 15.6mm has been recorded only 6mm inhibition zone of when combination with AgNPs 0.1 mg ̷ ml, that refer to antagonistic effects. Whereas optochin OP, penicillin P, and cephalexin CN antibiotics recorded in different effects.   Finally, with this study report. It can be concluded that AgNPs prepared from   Enterobacter aerogenes showed good antimicrobial activity when combined with antibiotics against Multidrug resistance Streptococcus mitis isolates. Thus these AgNPs can be used as a wide application to treat many oral cavity diseases in the dental field.