Evaluation and Comparison of Serum Creatinine Assay: Enzymatic Method Vs Kinetic Jaffe’s Method

Abstract

The principal motive of this research is to measure the analytical performance and practicability of enzymatic method with Kinetic Jaffe’s method. The cross sectional study was carried on 100 serum samples of outpatient population.  In order to measure serum creatinine, with Beckman AU480 auto analyzer, transasia reagent was used in Kinetic Jaffe’s method whereas coral reagent was used in enzymatic method. Pearson correlation coefficient method was applied to estimate the degree of association between two quantitative variables. The method comparison between the Enzymatic and Kinetic Jaffe’s has shown regression coefficient of 0.99, which means both the methods correlate with each other. The intra assay and inter assay precision values were found to be within desirable level in both the methods. Hence, the experimental result had shown that both the methods of creatinine analysis were comparable with respect to performance and precision.