Characterization of Sinirhodobacter sp., and Bacillus zanthoxylifrom Bio methanation plant with emphasis on its plant growth promotion(PGP).

Abstract

Plant growth promoting bacteria have gained significant role in its application as an agricultural input. Since most of the isolates that were investigated for their plant growth promoting potential were sourced from various habitats (terrestrial and marine), the present study was designed to identify and utilize isolates frombio methanation plants, comprising of spent waste from production units (mainly marine algae).The isolates from the samples of Biomethanation plants were identified and screened for their majorpotential PGP activities to assess its suitable applications.. Sinirhodobactersp., and Bacillus zanthoxyli was observed to be non-hemolytic bacteria with positive results for N fixation and hydrolytic enzyme production. B.zanthoxyli exhibited the trait of solubilizing zinc and the zone of zinc solubilization index; zinc solubilization efficiency for the isolate was 3.5 &350% in (24 hours), 7.75 & 750% in(48 hours). Sinirhodobacter sp., and B.zanthoxyli produced 90µg/ml and 176µg/ml of Indole acetic acid (IAA). B.zanthoxyli showed the presence of orange halo zone formation in CAS agar medium that is representing positive siderophore production. Italsopossessed ACC deaminase activity by releasing 147.8µmoles/ml of α-ketobutyrate. The optimum pH of both the isolates wasobserved to be 7.00 and optimum temperature for growth was observed to be 37°C for Sinirhodobacter sp., and at 30°C for B.zanthoxyli. This identification of two new less studied bacteria could pave way in developing novel value added plant growth promoting products.