Determination of a Suitable Concentration of Sodium Hydrophosphate for Storing Red Blood Cell Mass

Abstract

Background: Donated blood and blood components are currently used in the treatment of patients to help replenish the blood cells and circulating blood volume lost due to blood loss. Therefore, there is a need for effective preservation solutions for the storage of blood components, especially red blood cells.

The aim of the work was to identify changes in red blood cell mass characteristics up to 42 days of age in sodium hydrophosphate solutions with different concentrations.

The relevance of the work for Vietnam is the need to develop its own solutions for the preservation of red blood cell mass instead of the total use of imported ones.

Materials and Methods. Six different types of solutions were studied, labelled from D1 to D6, which corresponded to increasing concentrations of sodium hydrophosphate from 0.1 to 0.6 g/100 mL of the preservative solution in 0.1 g increments. Donor blood from healthy volunteers was processed according to the developed study model. A haematologyanalyser, an osmometer and a pH meter were used to estimate the necessary values of the tested red blood cell mass.

Results. The effect of sodium hydrophosphate on erythrocyte mass during long-term storage up to 42 days was detected. The solution with the lowest concentration in this study, sufficient for the required level of erythrocyte preservation parameters, was determined.

Conclusions. Sodium hydrophosphate is a suitable solution for the storage of red blood cell mass. When the cells are stored for 42 days at concentrations between 0.2 and 0.6 g/100 mL, it ensures that the cell count, pH level and osmotic pressure are maintained at an adequate level. At the same time, the D2 solution is the formulation with the minimum concentration to maintain the required level of the target parameters. Further studies are needed to investigate the activity of sodium hydrophosphate in combination with other components.