Efficiency of Hesperidin Against Liver Fibrosis Induced by Thioacetamidein Rats

Abstract

Hesperidin,a food derived antioxidants hase effect as chemo preventive agents in disease states involving oxidative stress, such as hepatitis. This study was done to clarify the  effect of Hesperidin (HES) in protecting the liver from the bad effect of Thioacetamide (TAA) . Forty-male Wister rats were divided randomly into four equal groups (10 rats in each ) and treated for 42 days as follow:Group I (control -ve) received normal saline 1 ml/kg BW, group II (control +ve ) received TAA 100 mg/kg B.W by i/p injection intraperitoneally (i/p), group III has received TAA 100 mg/kg B.W injection i/p plus HES 100 mg/kg B.W  orally, and Group IV received  TAA 100 mg/kg B.W injection i/p plus HES 200 mg/kg B.W orally. At the end of the experiment, all animals were weighted, sacrified for blood and liver samples collection. The results of this study showed  significant decrease (P<0.01) in B.W. and the level of SOD and CAT of the TAA treated groups in addition to significant  increase (P˃0.01)  in MAD levels. Although Hesperidin administration at doses 100 & 200mg/kg showed improvement  on these measurements, however 200 mg/kg was the more effective. The histopathological change resembled by excessive fibrous connective proliferation in group II were improved by HES 100 , 200 mg/kg B.W. In conclusion, HES counteract the hepatic oxidative stress induced by TAA in a dose of 200 mg/kg B.W