Biosynthesis of Silver Nanoparticles Using Phallusia Arabica and Evaluation of Total Antioxidant and Antibacterial Activity

Abstract

Biosynthesis of nanoparticles is a current field of nanotechnology which has eco-friendly and economic benefits compared to chemical synthesis. In this study, the ascidian Phallusia arabica ethanol extract was acted as a reducing agent for the preparation of silver nanoparticles (Ag-NPs). Ag-NPs was confirmed by the colour change using UV-Visible Spectroscopy and the size and shape of the NPs were determined by the SEM and the functional groups was identified by using Fourier transform infrared spectroscopy. Synthesized Ag-NPs were characterized by X-ray diffraction studies and EDAX. Atomic force microscope measurements confirmed the size and morphology of the synthesized Ag-NPs. The antibacterial activity of synthesized silver nanoparticles was tested against five pathogenic strains Bacillus cereus, Bacillus subtilis, Staphylococcus aureus, Enterococcus faecalis, and Shigella dysentriae