Greensynthesis and Characterisation of Silver Nanoparticles Using Phallusia nigra

Abstract

Biosynthetic technique is used for the production, characterization and application of silver nanoparticles (AgNPs), as reducing agent, which can be utilized in biomedical research and environmental cleaning applications. The reducing agents used to produce the nanoparticles were from the ethanolic extracts made from the simple ascidian Phallusia nigra. Prepared AgNP was observed by UV-Visible and IR spectroscopy. UV-Visible spectrum showed a peak as plasmon absorption in the range of 417 and 425 nm. AgNPs characterise their size and shape by Atom Force Microscopy (AFM). XRD pattern showed the characteristic Bragg peaks of (111), (200), (220) and (311) facets of the face center cubic (fcc). Antioxidants present in the ascidian reduce the Ag metal ions, for formation of silver nanoparticles and AgNPs presented in the ethanolic medium were quite stable, even up to 4 months of incubation. This work proved the capability of using biomaterial towards the synthesis of silver nanoparticle, by adopting the principles of green chemistry.