In Vitro Screening and Identification of Bioactive Compound Producing Marine Actinomycetes from Thoothukudi Coastal Water

Abstract

The coastal region water has been widely explored as the chief sources of microorganisms, especially actinomycetes, that are capable of producing a wide variety of bioactive molecules. The majority of the marine actinomycetes produce their value-added metabolites. The adversary effect of bioactive compounds of marine actinomycetes against various pathogens was pathetic. In our study, 10 different strains of actinomycetes were isolated from seawater. Among these two strains (MAT1 and MAT2) showed better inhibitory activities against the selected pathogens. Based on 16S rRNA gene sequence analysis this strain was most closely related to Streptomyces13581T (96.4 % sequence similarity) species and its phylogenetic analysis showed that the isolated strains were confirmed as Streptomycesarmeniacus and Streptomycesalkalithermotolerans. Itis one of the important alkaliphilicactinomycete strains. This work was analyzed for its physiological and biochemical characteristics of isolated actinomycetes and also to analyze the application of bioactive compounds in the biomedical field.