Micro Spectrophotometric Determination and Cloud Point Extraction of Metoclopramide with 4-Nitro Phenol in Pure Form and Pharmaceutical Drugs

Abstract

An eco-friendly procedure was developed for the detection of drug metoclopramide in pharmaceutical samples. It is a rapid and useful spectrophotometric method suggested for the determination of metoclopramide (MCP) with or without using cloud point extraction technique in pure form and pharmaceutical preparation. The first method was based on diazotization of the metoclopramide drug by sodium nitrite at 5 ºC, followed by coupling with 4-nitrophenol in a basic medium to form a purple colored product. To achieve this goal, all experimental variables for target analytes were previously optimized. The product was stabilized and its absorption measured at 440 nm. Beer’s law obeyed in the concentration range of (1-12) μg∙ml-1. Sandell’s sensitivity was (0.02 )μg∙cm-1, the detection limit was(0.0561 ) μg∙ml-1, and the limit of Quantitation was(0.0782 ) μg∙ml-1.The second method was the cloud point extraction (CPE) using Triton X-114 as a surfactant. Beer’s law obeyed in the concentration range of (1-12) μg∙ml-1. Sandell’s sensitivity was(0.0163 ) μg∙cm-1, the detection limit was(0.0331 ) μg∙ml-1, and the limit of quantitation was(0.054 ) μg∙ml-1. All variables including the reagent concentration, reaction time, color, stability period, and mole ratio were studied to optimize the reaction conditions. The mole ratio for the composition of the product is (1:1). Both methods were effectively useful for the determination of metoclopramide in a pharmaceutical dosage form. The attained results were in good agreement with the official and other methods in the literature.