TO STUDY THE ANTIMICROBIAL SUSCEPTIBILITY PATTERN OF PSEUDOMONAS AERUGINOSA AND DETECTION OF METALLO BETA LACTAMASE PRODUCING STRAIN WITH SPECIAL REFERENCE TO blaNDM-1 AND blaVIM

Abstract

The present study was 100 isolates of Pseudomonas aeruginosa from various clinical samples were included in the Study. Antimicrobial susceptibility testing by Disc diffusion method was performed in accordance with CLSI guidelines. Strains that were resistant to meropenem/imipenem are considered as carbapenem resistant strains. All the carbapenem resistant strains were further subjected to various phenotypic methods to detect MBL production. The phenotypic methods employed were Modified Hodge Test, Combined disc diffusion test and Etest. E-test gave better results than Modified Hodge test and Combined disc diffusion test. Conventional PCR technique is employed to detect blaNDM and blaVIM. blaVIM was present in 45.8%(n=11).