Isolation of Lytic Bacteriophages to Bacteria Isolated from Infected Skin Burns

Abstract

     In this study, 30 bacterial isolates from burn patients were enrolled (10 isolates from each of Acinetobacterbaumanii, Pseudomonas aeruginosa and Klebsiellapneumoniae), along with various crude samples for phage isolation collected from different environmental sources in various regions in Baghdad from June 2020 to November 2020. Each bacterial isolate was subjected to a full set of diagnostic and antibiotic sensitivity tests by using the Vitek 2 compact system and Kirby-Bauer method; in addition, matching lytic phages to the diagnosed bacteria were isolated after tested byPhage spot lysis assay. The results showed that all bacterial isolates involved in this study were highly resistant to most antibiotics, and some of these isolates resistant to all antibiotics used in this study.Four lytic phages were isolated against A. baumannii isolates, also four lytic phages were isolated against P. aeruginosa isolates and three lytic phages were isolated against K. pneumoniae isolates from sewage, farm soil, wastewater, faeces of sheep and chicken litter.  Finally, we proved the presence of lytic phages in the phage cocktail by using transmission electron microscopy.