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In this study we screened the 5077 samples collected from patients who attended SreeBalaji Medical College and Hospital, Chrompet, Chennai from Jan 2014 to Jan 2015 for proteus species. We found that the prevalence of proteus species to be 4.35%. Males were more vulnerable than females in acquiring Proteus infections. Higher prevalence of proteus fell in the age group of 15-40years at 6.33%. Results showed that the greatest number of Proteus Spp isolates from clinical specimens were from pus representing 5.3%. Proteus mirabilis was the most frequent species isolated in the entire specimen 64.6%. Thirteen different antibiotics representing different families of antibiotics were tested on Proteus Spp infections. The proteus specimens were highly sensitive to Meropenem and were highly resistance to Ampicillin. All cefoxitin resistances (85 samples) were taken for Amp C beta lactamase screening, from these 12 samples confirmed to be an Amp C positive by Amp C disk test. Meropenem is superior to other antibiotics for the treatment of infection due to AmpC beta lactamase producing Proteus species.