Downregulated BCL-2 and Caused Apoptosis in the (MDA-MB-231) Breast Cancer Cell Lines via Natural Compound (PCAE) That Majority Founds in the Clausena Excavata

Abstract

Propyl–cannabinol allyldimethylsilyl ether (PCAE) is one of the natural compound that majority founds in the Clausena excavata plant that belong to the Rutaceae family has used in the medical treated especially in the Asia. The compound under study is presumed to have anticancer effect against cancer cell lines. The mechanism action of compound is still exactly unknown. This study designed to evaluate the effect of (PCAE) on (MDA-MB-231) breast cancer cell lines through induce caspase 9, 3 and depressioin BCL-2. Western blot and mRNA expression assays were studied. The results showed clearly increasing in level of caspase 9 with decreasing in upregulated of BCL-2 and slightly increasing in the caspase 3. In conclusion, PCAE is a potent anti-cancer agent that can used to fight cancer.