Using Acetonitrile for Extraction of Aflatoxin from Aspergillus flavus under the Effect of Static Magnetic Field

Abstract

This study is conducted to minimize (aflatoxin B1) concentration extracted from A. flavus using acetonitrile under the effect of (M F.). The fungus (A. flavus), was expose to the north pole of the magnate, and then compared effect with the control treatment. (without a magnetic). The substrate utilized for the development of the organism (peanuts, cotton seeds and rice) were cleaned 1by autoclaving 6A static, magnetics field of (100) gauss is exposed to the (A. flavus) for (6, 12 and 18) days of aging at (28 °C) and, 25% dampness content. The convergence of (aflatoxin B) has been estimated by Enzyme Linked Immunosorbent .Assay (ELISA)..The substrates were grounded. If need and blended in with a particular measure of acetonitrile, at that point sifted, through Whatman,, channel, paper No1. .The concentrate, was tried for the aflatoxin B1 focus. Also, the outcomes were genuinely investigated. The outcomes showed that the north pole fundamentally diminished the aflatoxin B1 focus for the cotton seeds during (6, 12and 18) days,, which were (0.525, 0.475 and 0.368) ppb individually. The northern pole likewise essentially diminished the aflatoxin B1 fixation for the peanuts for the two time frames (6and 12) days which were (0.234and 0.301) individually yet there were no huge contrasts in the last time frame (18days) as contrasted and past periods just as and here was no huge contrasts at this period(18 days) between the control and north pole treatment. While for the rice the northern pole essentially diminished the aflatoxin B1 focus for the three time frames (6, 12 and 18) days which were (0.159, 0.032 and 0.024) ppb individually. This investigation was exhibited that there are critical impacts of the static attractive field in limiting the aflatoxin B1 fixation created by A. flavus .