Molecular profile of mecA, tst-1, Hla, Hlb, eta,etb,Erma, and Ermb virulence genes in Staphylococcus aureus using RAPD - PCR .

Abstract

The present work highlights the molecular typing of  thirty Staphylococcus aureus clinical strains isolated from skin lesions of  patients, consulted a dermatologist at Diwaniyah Teaching Hospital in Iraq,  using RAPD – PCR to detect of  eight virulence genes (mecA, tst-1, Hla, Hlb, eta,etb, Erma, and Ermb).  Tested strains gave the profile of St.aureus, deduced from yellow colonies on mannitol salt agar , and oxacillin (methicillin) resistance( MRSA).  The occurrence of virulence genes eta, etb, Hlb, Erma, and Ermb among the thirty  pathogenic Staph.aureus  were 16.66 , 50 ,60, 16.66 and 26.66% ,respectively. The RAPD-640 PCR could categorize the thirty  clinical strains under study into 19 groups strains (19 clades), regarding the obtained  DNA banding profile in each profile. The present data would underpin that Staph.aureus MRSA is the most predominate pathogen among patients with skin lesions .