Determine Some Mutations in the Foxp1 Gene in Autistic Patients in Baghdad Governorate

Abstract

Current study targeted 40 autistic children and 10 healthy as a  control group ranging from 1 to 12 years old, in Baghdad governorate from children referred to the Central Pediatric Teaching Hospital during the period from January  2019 to July  2019 . The amplification of the targeted region (730 bp) for foxp1 gene was performed by PCR reaction, which are in 40 autistic patient severe speech difficulty and 10 control. Agarose gel electrophoresis was used to confirm this amplification. The results of DNA sequencing for region (end of intron 16 and the beginning of exon 17 of foxp1 gene with length of segment 730 bp and a sequence of nitrogen bases by a Pioneer company/ Korean for 20 autistic patients and 5 control. 17 samples with 5 control of DNA sequence for this region appeared to have 100% identity with an expect value of (0.0), meaning that the sequence of DNA in these samples was completely matching the original reference sequence. There were no deletions or insertions in these samples. The sample A and B detected substitution mutation were Transvertion at Location 611606 by Nucleotide A>T in intron region.