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This research was conducted to prevent oral disease by preventing the recurrence and re-adhesion of germs present in toothbrushes by promoting sterilization of toothbrushes easily and conveniently at home using microwave ovens. Microwave treatment time was treated with a toothbrush contaminated with S.mutans for 0, 30, 60, and 90 seconds, respectively. To check the sterilization effect in the toothbrush, 1 ml of D.W was treated and 900 ?l was added to the agar plate. The processed agar plate was cultured at an incubator of 37C for 24 hours and the number of microorganisms was measured using CFU method. A total of three repetitions were performed in the same way to reduce the margin of error of the test results.S.mutans bacteria, which are highly resistant to sterilization, are mostly extinct in 90 seconds, it is believed that the household can kill microorganisms that reside in the Old River economically and effectively in a short time.Through this study, microwave sterilization effect is known, and the toothbrush sterilization is performed easily and conveniently at home to prevent the inflow of bacteria present in the toothbrush into the oral cavity, which is believed to be a suitable method to prevent oral diseases.