Molecular Diagnosis of Ocularmyiasis Causative Agent in Diwanyia City and Study of Epidemiological Data about infection Distribution in The City

Abstract

This study has aimed to diagnose the etiological agent of ophthalmomyiasis in Iraq in diwaniya city exactly as so as was aim to obtain epidemiological data about cases ratio and distribution in the city and affiliated areas, so that 30 samples of first instarlarva from infected cases have been collected from the eye by mechanical removal with ophthalmologist aid, then kept in ethanol 70%until used for DNA extraction andthen the extracted DNA used in PCR_RFLP. The results give only 20 samples were positive for osterousovis which also known as sheep botfly or nasal botfly, for epidemiological study have used the recorded data of cases in diwanyia educating hospital and hospitals of districts and sub-districts within the period from March to October 2020, the results showed that there is no significant difference in the number of cases among different ages, the results also showed that male cases are more than female cases. The study also has noted that most of the cases were recorded in villages more than city center due to shepherds and farmers presence. In addition to cases in spring were higher than summer and autumn seasons.