Anticancer, Cytotoxicity and Antimicrobial of Zinc Oxide Nanoparticles

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Thaer Ali Hussein, Mohammed Hassan Flaih, Munther Kamil Oudah

Abstract

Zinc oxide nanoparticles (ZnO-NPs) are versatile inorganic metal oxide nanoparticles known widely, which use in a broad spectrum of the applications. In this study, chemical method was used to prepare ZnO-NPs.The shape and size of the prepared nanomaterial were visualizedbya transmission electron microscope (TEM). The average length and diameter of the prepared ZnO-NPs were approximately100-200 nm and 25-50 nm, respectively. In vitro, the prepared ZnO-NPs were used as an anticancer agent toward breast cancer cell lines (MCF-7). However, the results of MTT assay showedthat ZnO-NPs is a promising drug against MCF-7. The prepared ZnO-NPs was also tested as an antimicrobial agent against (gramnegative and gram positive) bacteria. Escherichia coli (E. coli) and Staphylococcus aureus (S.aureus) were selected as a test ofbacteria. The antibacterial profile of the prepared ZnO-NPs was also studied with different concentration of ZnO-NPs with different methods such as a disc method, well diffusion agar method, minimum bactericidal concentration (MBC) and minimum inhibitory concentration (MIC).Moreover, an effect of time with different concentrations of Zn-ONPs at the growth of S. aureusand E. coliwas studied. Five different concentrations of ZnO-NPs were tested to determine the MICthatincludes 15, 10, 5, 2.5, 1, and 0.5mg/ml. The MIC valueswere found to be 1 and 0.5 g/m for E. coli and S. aureus, respectively. The resistivity against gram-positive bacteria wasless as compared with gram negative bacteria. IncreasingZnO-NPs concentration was relatedwithhigh antibacterial activity of ZnO-NPs. The antibacterial effect of ZnONPs was depending on a time and the effect was gradually.

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How to Cite
Thaer Ali Hussein, Mohammed Hassan Flaih, Munther Kamil Oudah. (2021). Anticancer, Cytotoxicity and Antimicrobial of Zinc Oxide Nanoparticles. Annals of the Romanian Society for Cell Biology, 901–909. Retrieved from http://annalsofrscb.ro/index.php/journal/article/view/2518
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