Detection of CTX-M and Carbapeneme Hydrolyzing Beta – Lactamase KPC in Clinical Isolates of Klebsiella Pneumoniae

Abstract

    The aim of this study was to detection Ctx-M and Kpc genes in clinical isolates of Klebsiella pneumoniae . it was isolated 54 isolates of Klebsiella spp. from different clinical specimens ( urine, stool, sputum , wounds , ENT and burns) that collected from different hospitals in Al-Nasiriyah city , Thi-qar province , Iraq. During period from September 2019 to February 2020. The isolates were identified and diagnosed by microscopic examination , biochemical tests and confirmed by API 20E and Vitek 2 system .Klebsiella isolates were tested against 18 antibiotic disks using the disk diffusion method. The results showed a high sensitivity of imipenem and amikacin, at a rate of 83%, for both antagonists,most isolates showed high rates of resistance to cephalosporins especially ceftriaxone (100%) and  showed high resistance for the penicillins group (Ampicillin, Amoxicillin-Clavulanic acid), it was recorded 98% and 96% respectively. Two methods were used to detect ESBL, 32(59%) isolates were producers of ESBLs with the screening test. Whereas 35(65%) of isolates were ESBLs producers by modified double –disk synergy test (DDST) . The results of the molecular diagnosis of the bla-CTX-M and bla-KPC resistance genes showed the presence of the bla-CTX-M gene in 31 isolates (58.4%) while the bla-KPC gene was not present in any isolate of  Klebsiella pneumoniae.