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The objective of this experimental research was to determine the immunity detection capacity for Escherichia coli in people at risk in Kerbala Province, against a standardized Escherichia coli antigen under laboratory conditions. The antiserum was obtained by inoculation of the inactive antigen of bacterial suspension of E. coli to rabbits breed Iraq. The serum with antibodies was obtained 28 days after starting the antigen inoculation program, in the respective specimens. Verifying the presence of antibodies, by means of the agglutination reaction in the 10 dilutions (1:10 to 1: 5120), with the respective antigen. The confronted antigen, with the serum and blood of people, was obtained agglutination. Concluding that 91.7% of the people under study have generated antibodies to this isolation.