In Vitro Assessment of MDS-derived Microvesicles on Umbilical Cord Hematopoietic Stem Cell Phenotype

Abstract

Introduction:

Myelodysplastic syndrome (MDS) is a heterogeneous group of hematologic clonal malignancies. As MDS cells transmit microvesicles in the bone marrow microenvironment along with other cells, the aim of this study was to investigate the effects of MDS bone marrow-derived microvesicles that have the ability to change the phenotype of a healthy hematopoietic stem cell to a leukemic-like phenotype invitro.

Method:

The bone marrow sample of MDS patients and healthy individuals was collected to prepare microvesicles and hematopoietic stem cells were identified as target cells.After culturing hematopoietic stem cells in the presence or absence of prepared microvesicles for 7 days, stem cell markers including CD34, CD90, CD38 and CD117 were detected and colony formation ability was observed. In addition, Cell survival, changes in microRNA-21, 10a, 181a / b expression and specific markers of leukemic stem cells including CD32, 25, 45RA were performed detect the leukemic-like phenotype.

Results:

In this study, healthy umbilical cord hematopoietic stem cells exposed to MDS bone marrow microvesicles for 7 days showed changes not seen in the other study groups. These changes, which include increased cell survival, increased expression of microRNA-21 and microRNA-10a, were observed while the stem ness of hematopoietic stem cells was preserved.

Discussion:

In this study, MDS-like changes in target cells were investigated and observed. It is also recommended that the effect of MDS microvesicles on hematopoietic stem cells be investigated for more than 7 days to show changes that require more time.