Augmentation Cystoplasty Using Autograft of Thigh Fascia Lata: Experimental Study in Canine Model

Abstract

Objective:The aim of this study was to evaluate the compatibility, enhancement of healing and late complications of autogenous fascia lata graft in the treatment ofexperimentally induced defects on the urinary bladder in dogs.

Method:Twelve adult healthy local breed male dogs were used. Animal was anthesized by atropine sulphate 0.03 mg/kg. B.W. as a premedication and 10 min later a mixture of ketamine hydrochloride and xylazine hydrochloride injected I\M at dose of 15 and 5 mg/k.g B.W. respectively. Under aseptic technique fascia lata was harvest from lateral surface of thigh, then celiotomy performed to exposed urinary bladder (UB) and induce 2×3 cm defect in ventral surface of it then substituted by 3×4cm patch of fascia lata and fixing by simple continuous suture pattern 3\0 polydioxanone.

Results:There were no death related to the procedure, all dogs voided spontaneously after surgery, urinary frequency and animal behavior changed observed through the first threedayspost-grafting includingoliguria and increase urinefrequency,loss appetite and less depress also noticed. Macroscopically good tissue integration was observed between graft and surrounded bladder tissue with gradual decrease in graft sizewith progress of study, bladder intraluminal stone was not recognized in this study. Histoopathological picture at 2^nd week post graftingshowed well incorporation between fascia lata graft and host tissue throughgood infiltration of inflammatory cell, fibroblast and myofibroblast, as well as active  neovascularization with complete mucosal urothelium comprised of transitional cells, while at 4^thweek showed deposition of dense collagen that replaced the fascia late in several section that attached mostly with host muscle fibers,thickening in epithelia was showed at 8^th week and underlying well developedby newly muscle regeneration within fasciatissue, smallfragmentof fascia lata still showed at this time.

Conclusion: Autograftfascia lata can be successfully used for replacementsmall defect of (UB) without any local or system complications in dogs.defect in