Identification of Corona Virus Infection in People Infected with SARS-CoV-2

Abstract

Localization and eradication of the COVID-19 pandemic requires that a number of challenges are addressed, and diagnosis is the first of these challenges. Establishing diagnosis can not only detect ill people, but also create a statistical database, which may help manage containment measures, realize epidemic-prevention measures and organize the work of the world community on the way to elimination of the emerged problem.

Study of the methods of COVID-19 diagnosis constitutes the aim of our research. By means of analyzing literary data and methods of laboratory studies we have managed to present variants of reactions use (polymerase chain reaction, enzyme linked immunosorbent assay) for establishing the COVID-19 diagnosis.

The authors of the article have conducted the analysis of COVID-19 diagnosis protocol files valid in the People’s Republic of China, the United States of America, South Korea, the Russian Federation and Ukraine. Variants of polymerase chain reaction setup were studied (diagnostic identification of one or several viral genes). The mechanism of diagnosis in case of SARS-CoV-2 viral infection and variants of use of corresponding methods of laboratory studies are presented.

Polymerase chain reaction (PCR) remains the only diagnostic reaction acknowledged by the World Health Organisation, however, a range of laboratory and instrumental methods can be used as auxiliary, helping lower the load on PCR diagnostic laboratories. There is no protocol of PCR administration, and determining the optimal diagnostic procedure of COVID-19 diagnosis is one of the forward-looking tasks for the world academic community.