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The adolescence is an age group where the not only body faces drastic changes in it but its essentials requirements also increases. It requires proper balance of rich diet, lack of which may lead to condition of anemia. In todays scenario, it is one of the most common condition among adolescents especially girls. This study aims to evaluate the combinatorial effect of folic acid, vitamin E, vitamin C and Zinc with iron when administered in anemic condition by adolescent girls. The results have showed remarkable effects of these combination among subjects before and after supplementation. The iron profile parameters including ferritin, total iron and level of haemoglobin showed a significant percent increase among the subjects. The results revealed trans sat. level increased by 18.550 4.156 (%) in case group while 4.948 1.941(%) in control group. A similar effect was traceable in other parameters too with remarkable increase after supplementation of vitamins deriving the inference that combined effect of iron tablets is much more than its effect alone. Iron was seen percentage increase of 29.883 16.069 (%) (case group-group II) much higher to 15.361 2.020 (%) (control group- group I). The Ferritin level also showed highly significant results with percentage increase in the level by 52.844 36.041(%) (group I) and 7.187 6.370(%) (group II). Results indicated a significant correlation when before and after vitamin supplementations were compared. Thus, the combination could prove beneficial if administered in anemic condition to the adolescents especially girls.