Phenotypic and molecular identification of blaOXA ESBLs gene in Escherichia coli isolates from UTIs patients in the Al-Basrah province, Iraq
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Abstract
The emergence of antibiotic-resistant microbes, including urinary tract infections, poses a significant threat to public health, making treatment increasingly challenging. Between 5th January to 22nd February, current study relied on 200 urine samples were collected from patients at Al Sadr Teaching Hospital in Al-Basrah Province, Iraq, who were suffering from urinary tract infections (UTIs). A result of current study found that 71 (35.5%) of urine samples from urinary tract infections patients showed positive bacterial growth. The growth was distributed to 47 (66.2%) Escherichia coli and 24 (33.8%) of other Gram-negative species. The diagnostic gene 16S rDNA by PCR method the results showed that all 47(100%) E. coli isolates. Furthermore,the double-disc approximation method (DAM) showed positive results in 44 (93.6%) E.coli isolates that produced ESBLs. While double-disc synergy test (DDST) showed positive results in 13(27.7%) of E.coli isolates. The study also detected Extended-spectrum β-lactamses (ESBLs) genes by PCR, amplifying the blaOXA gene in E.coli isolates. The amplified genes' bands were characterized at approximately (619 bp) for blaOXA and compared to the standard molecular DNA ladder at 2000 bp. The results showed that all 47(100%) E.coli isolates gave positive results for the blaOXA gene.