Gene expression of Entamoeba histolytica in diarrhetic patients from Wasit province

Abstract

In order to identify the amoebiasis-causing Entamoeba histolytica in people who were suffering from diarrhea, this study made use of molecular detection technologies. We gathered stool samples from one hundred people who were experiencing symptoms of diarrhea and classified them according to their age and gender. The findings of an inspection using a direct microscope showed that ninety percent of the samples had entamoeba germs. According to the findings of a microscopic inspection, the rate of infection with Entamoeba was much greater in females (86.73 percent) than in men (70.8 percent), and this difference was significant at the level of 0.05 for the P value. The investigation also indicated significant infection rates in the age categories of 5-11 years and more than 50 years (respectively 85.23 percent and 78.81 percent), but a reduced infection rate in the age group of more than 2 years (43.45 percent). The nested multiplex polymerase chain reaction, in which the small subunit gene was used, was able to establish that the infection rate for E. histolytica 91 was greater (91 percent). The prevalence of the Entamoeba histolytica infection is much higher in females (89.23 percent) than it is in males (82.35 percent). Both the older age group (>2) and the younger age group (28-48 years) had an infection rate that was one hundred percent attributable to E. histolytica. Based on gender, age, and the results of nested multiplex PCR, this gave the proportion of people that were infected with Entamoeba histolytica.