Studying the Effects of Active Compounds in Clove Plant and their Impact Against Certain Types of Candida Species

Abstract

The study included the detection of active compounds by using the high-performance liquid chromatography (HPLC) method.  It revealed about 7 effective compounds, as the clove plant contains volatile oils and the most effective and concentrated compound was Eugenol acetate. The genus Candida spp. was identified based on cultural and microscopic characteristics was revealed in a way of smooth, shiny, convex colonies, white to milky in color, and distinguished by its distinctive odor.  The shape of the cells appeared spherical to oval or longitudinal, single and budded. The virulence factors of Candida species were studied Its ability to produce Protease enzyme and the possibility of isolation from yeast C. albicans on the enzyme production, followed by C. tropicalis yeast while the other species do not have the ability to produce the protease enzyme. The ability of Candida was to produce the hemolysin enzyme especially the two species C. albicans and C. glabrata have hemolytic ability, while other isolates portability was non-hemolytic, and their ability to produce phospholipase enzyme was demonstrated by the isolate of C.albicans which recognized by the  appearance of a white dense area around the growing colonies, which is the area of   deposition on the medium of  solid egg yolk, while the rest of the isolates did not produce the enzyme phospholipase. The alcoholic extract of Cloves plants was prepared for the purpose of identifying the antagonistic activity against Candida species, it was noted that the clove plant inhibited all species.