Effect of Different Media, Temperature, PH, Carbon Source and Nitrogen Source on Mycelial Growth of Lasiodiplodia Theobromae Causing Crown Rot of Banana.

Abstract

In this study, the effect of different media, temperature, pH, Carbon source and Nitrogen source was tested against growth of Lasiodiplodia theobromae under in vitro conditions. Among the solid media tested, Potato Dextrose Agar Medium was most suitable for the growth of L. theobromae which recorded 90 mm mycelial growth. Among the different liquid media tested, Potato dextrose broth found to be most suitable which recorded maximum mycelia dry weight (231.52 mg). Maximum radial growth (90 mm) and mycelial dry weight (327.43 mg) recorded at 30^oC. pH 7.0 was found to be most suitable which is recorded maximum radial growth, Maximum mycelial dry weight and good pycnidial production. Among tested carbon sources, dextrose recorded the highest mycelial growth (90 mm) and the highest dry weight of (371.60 mg) with excellent pycnidial production. Among the tested nitrogen sources, peptone recorded the maximum growth of mycelium of about 88.66 mm and mycelial dry weight of about 364.76 mg.