Use of Genetic Methods for Detection of Streptococcus Pneumoniae Virulence Factors Isolated from Patient with Pneumonia

Abstract

This study  aimed to detection of Streptococcus pneumoniae and detection of their virulence factors , For this purpose 60 sputum samples   were collected from  patients with bacterial pneumonia all patient arrived to critical care unit (CCU). Bacterial culture technique and genetic technique were applied. The result showed that  Streptococcus pneumonia detected in rate of 10% . results of mPCR showed that the PlyA&LytAdetected in rate of 100% while PsaAdetected in rate of 71.4%.