Synthesis and Anti-Microbial Activity of Novel Substituted Chalcone Derivatives

Abstract

Some chalcones derivatives  show antimicrobial, anti-fungal and insecticidal activity. All solvents were redistilled before use. Reactions were routinely monitored by thin layer chromatography and spots were visualized by exposure to iodine vapour or UV light. All the synthesized compounds were purified by recrystallization. Melting points were determined by using open capillary method. Fine powdered zinc chloride (8.25) was dissolved in glacial acetic acid (18ml) by heating on sand bath then dry resorcinol (appx.5.5 gm) was added with continuous stirring to the mixture at 140^oC. The solution was heated until the solution just begins boil and kept it for 20 min at 150^oC temperatures. All the compounds synthesized in the present investigation were screened for their anti-bacterial activity by Cup plate Method. Broth double dilution method was use for screening Antifungal activity. Ketoconazole used as standard, was dissolved in sterile DMSO. DMF, DMSO were also test as control. All the compounds C1-C6 were evaluated for antimicrobial activity against gram positive bacteria, Staphylococcus aureus, gram negative bacteria Escherichia coli, and fungi Candida albicans. compounds C3 and C4 have near to activity significant with standard compound amoxiciliine and  Compound C1, C3, C5 shown growth of fungus in petridish other compounds shown no growth. According to docking studies compound C1 shown as maximum negative contribution -8.03006 and C6 shown less contribution -7.71101.