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Endophytes were isolated in culture from the surface of water conduct photosynthetic leaf tissues during six month continuous growing seasons. A total of 33 representing species were recovered from a total of 600 (every month/100 bits) plant leaves tissues segments of musk grass; stonewort in the submerged aquatic plant of Chara delicatula. Although isolation frequency was low, endophytes were phylogenetically diverse groups and species-rich. Compactly among the six months, a continuous study in leaf sampled and a pond revealed that frequency isolation and diversity did differ significantly between collection periods, among species are slightly varied or in the maturation, between leaves ages. These plants were collected from a fresh-water pond in winter season at Mannargudi in Tamil Nadu. Among these three belongs to Ascomycetes. Five fungal species belonging to Coelomycetes and twenty Hyphomycetes beside to five sterile forms. Endophytic fungi were studied shows that some of occupying the leaves tissues.