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Water is a finite and vulnerable resource. Consumption of polluted water puts lives and livelihoods at risk because water has no substitute. There are many ways in which water gets polluted. These include wastes from industries like mining and construction, food processing, radioactive wastes from power generating industries, domestic and agricultural wastes and by various microbiological agents. Water pollution is a serious threat all over the world in the light of limited fresh water availability and increasing generation of waste water being discharged either on land or into rivers, lakes, water bodies or sea thereby polluting the fresh water available in rivers, lakes, water bodies, and groundwater. The water pollution scenario in India is equally critical posing great threat to human health, aquatic life, vegetation and ecological balance. In this paper, the effects of polluted lake water and analyzed by various analytical tests that this lake water was highly contaminated by the open defecation activities and mixture of domestic wastes which has been confirmed by the presence of microbial agents like Escherichia coli and Staphylococcus aureus hence it’s not suitable for human consumption has been proved.