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In this research paper, we present a simple and eco-friendly biosynthesis of silver nanoparticles using Chromolaena odorata leaf extract as a reduction agent. For phytochemical screening such as carbohydrates, terpenoids, resins, saponins, tannins and alkaloids in the Chromolaena odorata extract, the current research was conducted using normal protocols and antibacterial activities of the silver nanoparticles extract of Chromolena odorata leaf prepared from extracts. The phytochemical constituents of C.odorata were qualitatively analyzed and the occurrence of different phytochemicals in the ethanol extract was verified. ZETA SIZER, FTIR, SEM, AFM, XRD and UV- Visible spectrophotometers were used to study the characterization of silver nanoparticles. AgNPs of chromolaena odorata leaf extracts were used for antibacterial activity against four bacterial species, namely E.coli, Staphylococcus aureus, Pseudomonas aeruginosa and Bacillus subtilis. Good findings for sugars, terpenoids, tannins and saponins were seen in the leaf extract. Silver ion reduction in the presence of plant extract and the production of silver nanoparticles confirmed a transition from light black to dark black in the color of the solution after treatment with AgNO3.FTIR leaf extract spectrum, clearly displaying maximum peaks at 803.77 cm-1 corresponding to C-C stretch of Alkane, peak at 1243 cm-1 assigned to C-O stretch of Alcohol, peak at 2119.047 cm-1 assigned to C=C stretch of Alkynes terminal, peak at 3051.265 cm-1 corresponding to C-H stretch of Aromatic and relatively strong peak at 3700 cm-1 attributed to Alkynes free O-H stretch, peak at 3051.265 cm-1 corresponding to C-H stretch of Aromatic and relatively strong peak at 3700 cm-1 attributed to Alkynes free O-H stretch, peak at 3051.2655 cm-1. The highest absorbance is found to occur at 190nm. The morphology of the particle and its size were determined by SEM. It is remembered that the shape was structurally spherical. It varies in size from 93-191nm. AFM was able to see the peculiar shapes and the composition of the silver nanoparticles. The XRD spectrum showed different peaks of around 390. The good organism resistant to Chromolaena odorata AgNPs was found to be Pseudomonas aeruginosa, while others showed intermediate quality. It was discovered that the silver nanoparticles synthesized from C.odorata extracts had the strongest antimicrobial efficacy against P. aeruginosa (15 mm), S. (14mm) with aureus, and E. Coli (14mm) and, respectively, the lower antimicrobial activity of silver nanoparticles synthesized by Bsubtilis (13 mm).