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duction: Different materials and agents have been introduced for removing the smear layer and bacterial endotoxins from the root surface. Hence in the present study we aim to evaluate four different root conditioning agents on the root surfaces.
Material and methods: Eighty freshly extracted human single rooted single canal teeth with periodontal involvement were selected for this study. Following the extraction, the teeth were thoroughly washed and root planing was done using gracey curettes. The teeth were decoronated using carborundum discs. The teeth were divided longitudinally by a water cooled high speed fissure bur. Apical thirds of all teeth were resected and remaining root was vertically sectioned longitudinally through the root canal. All pulpal tissue was thoroughly removed.A total of 80 specimens were prepared and divided into four groups.
Results: On examining the root surfaces it became clear that maximum smear layer was seen on the root surface rinsed with saline and the cleanest surface was the one cleaned with MTAD followed by doxycycline which was then followed by 17% EDTA.
Conclusion: Because of more persistent effectiveness on early root conditioning (chelating effect in less than one minute of applying), MTDA and Doxycycline are preferable root conditioner agents which could be used in regenerative periodontal therapies.