Phytosynthesis of Cobalt Ferrite Nanoparticles by Crude Extract of Rheum Ribes.

Abstract

The objective of this study was identifying the flavonoids in Rhizomes of Iraqi Rheum ribes and utilization these extract to phytosynthesis of cobalt ferrite nanoparticles with characterization of them. The results obtained from the HPLC analysis indicate that rhizomes of Iraqi R. ribes contain seven compounds of flavonoids which include: 1.735 μg/g aloe-emodin, 24.966 μg/g emodin, 5.533 μg/g chrysophanol, 17.542 μg/g physician, 4.242 μg/g rhein and 15.873 μg/g rutin,.

Plant extract-mediated cobalt ferrite nanoparticles were characterized by various techniques to analyze the size, shape, crystallinity.  Their average size (which calculated by AFM) were: (100.36, 68.80, 69.01) nm for (1, 2 and 3) procedures, respectively. While procedure 4 was fail to produce nanoparticles, their average size was (192.38 nm). From SEM study, it is confirmed that the particles are almost spherically shaped and uniformly arranged.

The FTIR results showed strong bands are observed in (procedure 4) around 585- 400 cm−1 confirm the presence of CoFe2O4 nanoparticles while other procedures showed tetrahedral metal stretching ν(Fe-O tetra)  and octahedral metal stretching ν(Co-O) bond.

The XRD patterns of all samples indicate spinel cobalt ferrite magnetic nanoparticles having face centered cubic structure. This structure was pure in procedure 1. The average crystallite size of nanoparticles was calculated by Scherer's equation, they were: (47.41, 45.1, 50.62 and 44 nm) nm. The results of UV–visible spectral of procedures (1-4) showed that the absorption spectra of phytosynthesis NPs exhibit strong absorption at 221 nm. More than one band gaps of each sample (3.4 eV and 4.5 eV). These two energy gaps are indicating to presence of a quantum well. These results follow quantum laws.