Effect of Repaglinide and Metformin as Anti-Diabetic Drugs on Fertility and Sex Hormonal Levels of Male Albino Rats

Abstract

Background : The weakness of  reproduction system in male that complaining with diabetes, by effects on the endocrine functions lead to depression of ejaculation, libido and erection in male, diabetes is associated to reduce ejaculated semen volume with decreased vitality and motility of the spermatozoa, with no change in sperm viscosity, the critical concern, as it is important to ensure diabetic individuals' reproductive health while on anti-diabetic medications. Therefore; this study  has  investigated  the effect of repaglinide and metformin as anti-diabetic drugs on mating ratio and sex hormones levels of albino rats

(2) Methods: in this study induction T2DM in 28 male rats by  injection of alloxan 3 doses of 120 mg/kg intra-peritoneal, and classified to 4 groups: 1-Control without any drug (positive control) 2-Treated by metformin (500 mg/kg)  3-Treated by replagnide (4 mg/kg) -4Treated by metformin (500 mg/kg) and replagnide (4 mg/kg), and control groups without diabetes include 28 male rats, and classified into same groups of drugs administration, after 50 days of gavaging, choice two rats from each group for test the sexual performance and fertility by mating each male with two female rats, and sacrificing other animals and isolated serum sample and frozen at (-20) until to biological  test (LH, FSH and testosterone)

(3) Results: The percentage of mating was performed 100% in diabetic rats among 3 weeks while the diabetic male rats treated with metformin and animals treated with repaglinide complete the mating percentage in two weeks, whereas the diabetic rats treated with mixture of met+repa performed100% of mating rates in first week,  The results revealed significant decrease (P>0.05) in mean of testosterone  and luteinizing hormone in the diabetic compare with non-diabetic rats, but no significant differences (P<0.05) showed in other groups of study. The estimation of follicle stimulating hormone levels in study groups revealed significant decrease (P>0.05) in the animal of diabetic group compared to non-diabetic rats, and in the  groups of animals treated by mixture of drugs (metformin and repaglinide) also observed significant elevation (P>0.05) in the diabetic rats compared to non-diabetic rats, while there was no significant differences (P<0.05) among other groups.

(4) conclusion: Mixture of treatments metformin and repaglinide have very positive effect on diabetic animals and enhancement sexual activity and restore sexual hormones level.