Effect of Repaglinide and Metformin As Anti-Diabetic Drugs on Epidydimal Sperm Parameters

Abstract

Background: Diabetes Mellitus type 2 comprises 90 - 95% of the total cases of diabetes worldwide, Because of the pandemic rise of diabetes and its high incidence among young boys, this is a critical concern, as it is critical to ensure diabetic individuals' reproductive health while on anti-diabetic medications. Therefore; this study  has  investigated  the effect of repaglinide and metformin as anti-diabetic drugs on albino rats sperm parameters(2) Methods: The hyperglycemia was induced by alloxan in male rat model after injection of alloxan 3 doses of 120 mg/kg intra-peritoneal was used for the induction of diabetes mellitus type 2. Fifty six of male rats was classified into two main groups, first group (28 rats) include 4 sub groups of male rats which treated by alloxan (DM inducer) each of them contain 7 rats:-1Control without any drug (positive control)2-Treated by metformin (500 mg/kg) 3-Treated by replagnide (4 mg/kg) -4Treated by metformin (500 mg/kg) and replagnide (4 mg/kg), second groups (28 rats) also include 4 sub groups but without alloxan treated and  each of them contain 7 rats, also classified into same first group and treated by same doses of treatments. After 50 days of gavaging and sacrificing the animals for test epidydimal sperms parameters. (3) Results revealed significant decrease (P>0.05)  in control diabetic group compared to non-diabetic in the levels of  epidydimal sperm concentration, significant reduction (P<0.05) in abnormal sperm morphology percent of diabetic group compare with non-diabetic all treated by mixture of drugs, and significant elevation (P> 0.05) of progressive motility percent in the group diabetic rats compared to non-diabetic rats which treated by mixture of drugs. (4) conclusion:Mixture of treatments metformin and repaglinide have very positive effect on diabetic animals and enhancement sperm parameters, but metformin increase abnormal morphology percent of sperms for non-diabetic rats and increase non-motile percent of sperm for diabetic rats.