Hormonal, Histological and Comparative Study an Effect of Pure Ginseng on Testicular Function during and out of Breeding Season of Rams in Basrah

Abstract

The study aimed to investigate the effect of Panax ginseng powdered supplemented 2and 5 gram as a diet on testosterone, SSH and ICSH  levels, on the other hand, Panax ginseng effect on some physical parameters of sperms, histological testis traits, and testicular size in and out of season in adultrams.This study was conducted at the laboratories of Theriogenology, Department of Surgery and Obstetrics, College of Veterinary Medicine, Basrah University. The experimental study extended from May 2019 to the end of December 2020. Twenty adult rams  (18-24 month old) were divided randomly into 3 groups; The 1st group 8 adult rams were subdivided into 4 rams fed 2 gram standard administered containing Panax ginseng, while the other 4 adult rams fed 5 gram standard administered containing Panax ginseng once a day for 90 days during the breeding season. The 2nd group 8 adult rams were subdivided also into 4 rams fed  2 gram standard administered containing Panax ginseng, while the other 4 rams fed 5 gram standard administered containing Panax ginseng once a day for 90 days out of season. The 3rd group 4 adult rams were used as the control group two times (in and out of season).The results illustrated the testosterone ,SSH and ICSH were high significant (P<0.05) values between treated groups of ginseng supplemented 2and 5 gram as a diet compared with  control group in and out of season .The physical parameters of sperms evaluation ( total sperms count, sperms motility, and progressive movement ) of this study showed superiority  improving of above parameters ,while the total immotile sperms andnon-progressive sperms movement showedlow significant (P<0.05) values in treated groups of ginseng supplemented 2 and 5 gram as a diet compared with control group in and out of season.The angiogenesis of the seminiferous tubules were  increased and the incensement of the numbers of spermatogonia, primary and secondary spermatocytes were observed in the Panax ginseng groups through a microscopic examination during and out of season.Values of  testicular diameter showed a highly significant difference (P<0.05) at (75 and 90 ) days in treated groups of ginseng supplemented  2 and 5 gram as a diet compared with control group in and out of  season. From the above mentioned results, we conclusion that Panax ginseng may affect the morphology and motility of sperms, the important factor in male fertility, and its antioxidant status in  rams.