Molecular characterization of Acinetobacter baumannii isolated from different clinical sources

Abstract

Acinetobacter baumannii is one of the most important multidrug bacterial types (MDR)opportunistic pathogenic, in addition to its high ability to gain resistance to various antibiotic groups, collected (25) from various clinical sources of infections: wound, burns, sputum , blood, Urine and csf . the distribution of isolations according to the sources of injury burn samples ( 7)% 28 and then the urine (4)%16, blood ( 4)%16, wounds (3 )12%, sputum (3)%12 csf (2) 8% .Antibiotic susceptibility testing varied (Tetracyclin, Pipercillin, Trimethoprim, Ceftazidime, Amikacin, Ciprofloxacin, Cefepime, Meropenem, Imipenem, Colistin) and by (100%,92%,92%, 96%,84%, 80%, 88%, 76%,56.60% ) respectively. The 25 isolations of A.baumannii showed a differentiation of two patterns according to their resistance to antibiotics, with 18 (72%) XDR isolation being resistant to(7-10)antibodies, while 5 (20%) of MDR isolationswere resisted for (3-6) antibodies. The genetic variation in this study was a description of the molecular properties related to the relationship of 10 isolations of A.baumannii bacteria andusing Randomly Amplified Polymorphic with RAPD DNA and 16sRNAtechnique where genetic DNA was extracted from 10 isolations from isolated A.baumannii from various local clinical sources. In addition, the genetic distance and cluster analysis between the various A.baumannii isolations were analyzed and estimated using upgma computer software based on10 genetic patterns distributed in two groups, 9 of which were similar to molecular weights and one single isolation ranging from(2000-100 base pairs)This study demonstrated genetic differences (polymorphisms of DNA) between the10 A.baumannii isolations isolated from different sources. Sequencing was conducted for five isolations taken from different local clinical sources, where the isolations showed a different variation and the evolutionary tree was painted, with MW599783, MW599779 and MW599782. Close to global isolations, MW599780 and MW599781 are close but far from the insulation MW599783, MW599779 and MW599782