A Study on the Antibacterial Effect of Chamaecyparis Obtusa Extract on the Causative Agent of Dental Caries

Abstract

We conducted to evaluate the antibacterial effect of the Chamaecyparisobtusa extract on Sreptococcusmutans (S. mutans). The strains used in this experiment were investigated for S. mutans. The activity of Chamaecyparisobtusaextract was measured the diameter (mm) of the clear zone by the paper disk (Φ8 mm) diffusion method and colony forming units (CFU). The significant difference among groups in one-way ANOVA and Duncan test.As a result, the ethanol extract of Chamaecyparisobtusa showed the strongest antimicrobial activity against S. mutans. The clear zone of S. mutans was found to be 14 mm, 15.5 mm and 17 mm at 20 mg/mL,30 mg/mLand at 40 mg/mL, respectively. In addition, the Chamaecyparisobtusa extract exhibited concentration-dependent inhibition of CFU over 1.25 mg/mL, with 100% inhibition at a concentration of 40 mg/mL. These results show that Chamaecyparisobtusa extract has anticariogenic effect on S. mutans. Therefore, Chamaecyparisobtusa extract can be used effectively in the prevention of oral diseases.