Localization of TNF-α in Cutaneous Incisional Wound Treate

Abstract

The interest on plants research hadbeen increased all over the world and now there is a large body of evidence about thepotential immense of the medicinal plants to be used in a variety of traditional systems Aim : Localization of TNF-α in Cutaneous Incisional Woundand evaluation the potency of ethanolic extract of Hibiscus sabdariffa L (flavonoids) on wound healing by a phytochemical, biochemical, histological and immunohistochemical method. Materials and methods: Twenty four adult New Zealand rabbitswere divided into control(12) and experimental (12)groups, incisional wound was created on facial skin and local application of FV extract will be done for experimental groups only ,all animals were sacrificed for 3,7 ,10 and 14 days, specimens were prepared for histological and immunohistochemical analysis. Results: As shown by examination of histological sections that healing was accelerated after flavonoid application and results of statistical analysis indicated that wound healing higher in 7and 10 days in experimental group than control group. Findings obtained in this study showed decrease in percentage of positively stainedTNF-α in 10 and 14 days. Conclusion:The ethanolic extract of H.Sshowed fairly goodwound-healing activity as demonstrated by histological results and TNF-αexpression by cells involved in the process in different durations.