Identification of GH gene Polymorphism using PCR-RFLP of Iraq and Belarus Population sheep breeds

Abstract

The Growth Hormone gene is confederated to be a major active gene in mass gaining for the improvement of meat industry production, Therefore, our study aims to identify the GH gene polymorphism that correlated with meat gain parameters and traits in the Belarus and Iraq sheep with the application of PCR-RFLP method and the use of the HaeIII enzyme for the digestion of the PCR product. As result, three genotypes were spotted (n= 150), two homozygous genotypes GH AA, GH GG and heterozygous genotype GH AG, with an actual frequency of 0.153, O.247 and 0.6, respectively. The obtained results aid meat production amelioration by controlling the expression of the identified genotypes of the GH gene.