Development and Characterization of Chicken Egg Yolk Antibodies Igy Against Porcine Pancreatic Lipase as a Preliminary Step to Control Obesity

Abstract

Obesity, once seen as a major clinical problem in high-income countries has been a significant health issue even in developing countries and urban populations in recent decades. Issues related toobesityincludinghypertension, cardiovascular issues are highly critical that it leads to the high mortality rate in most of the countries. Obesity observed in children is of greater concern to be treated with surgeries and drugs in younger ages leading to side effects in later years needs to be addressed very carefully. Hence the present study attempted towards antibody generation towards lipase enzyme which is significant in the clinical condition of obesity. Anti-lipase IgY chicken antibodies were developed generated in experimental white leghorn chickens and purified by water dilution method followed by the desalting procedure. Antibodies were characterized for total protein estimation by the Lowry method as 56mg/mL and by the Bradford method as 90mg/mL. Purity and the molecular weight of the porcine pancreatic lipase antigen and IgY antibodies were found to be 65kDa and 180kDa respectively. The antibody titer of anti-lipase IgY antibodies as determined by Indirect ELISA revealed the highest titer on the 63rdday of immunization with an increasing peak up to the 35th day of immunization. Hence the present study has opened up the idea of antibody therapy for treating obesity against working upon the key enzyme lipase with initial characterization of the generated anti-lipase IgY antibodies. Further experimental studies on animal models followed by human clinical trialsneed to be critically studied on the mechanism of action and effectiveness of these antibodies in controlling the enzyme activity and in turn the clinical condition of obesity.