The Effectiveness of a Biotechnological Method for Controlling the Content of Gossypol

Abstract

The results of studies devoted to the study of the occurrence of gossypol are presented; factors contributing to the formation of gossypol are listed. A new method for controlling the content of gossypol in cottonseed has been developed: it has been experimentally established that when cottonseed is soaked in a solution of water of cattle manure, lamb manure and poultry manure, and the catalyst, a decrease or increase in the content of gossypol is observed. When soaking cottonseed with residual fiber in the solution, the level of gossypol decreased to 0.063%. Experiments have shown that with a concentration of 21 kg of cattle manure, 21 kg of sheep manure and 13 kg of poultry manure, 25 g of urea and ammonium nitrate each in 14 liters of water, the process proceeds rationally towards a decrease in gossypol. The essence of the method for determining the quantitative content of gossypol consists in the extraction of free gossypol from the analyzed product with a mass fraction of moisture not exceeding 7% with anhydrous acetone and subsequent quantitative determination of gossypol by a photometric method according to existing standards. Due to the naturalness of the components of the solution, cottonseed oil obtained from cottonseed, processed by a biotechnological method, can be used as a healing one. Therefore, a new way of managing gossypol content is beneficial.