Main Article Content
This study aimed to investigate the antibacterial effect of Nanosilver on haematological and biochemical parameters in common carp, Cyprinus carpio infected with Aeromonas hydrophila. Silver nanoparticles (AgNPs) were prepared chemically from silver nitrate and biologically from lemon extract. The characterization of silver nanoparticle was studied using a UV spectrophotometer, TEM, SEM, and FTIR, which showed that the AgNPs was spherical shape, size 30-50 nm. Experimentally, 150 fish (40 ±5g( were divided into eight groups, all treatment groups except control negative group were infected experimentally with A. hydrophila by intraperitoneal injection (0.1 ml; 1. 43×10-7 CFU/ml) and were treated with different concentrations of AgNPs as follows; [chemical method:T1 0.125 g/ l, T2 0.25g /l], [biological method: T3 0.045 g/l, T4 0.075 g/l and T6 0.15g/l] and T5 treated with oxyteteracycline (OTC) (0.4 g/l). All groups were treated by bath treatment for 10 days for 30 min per day for 10 days. Results indicated that WBC count was significantly decreased in all treatment groups compared to C+ group. Lymphocyte percentages registered significant decreased in T4, T5, T6 groups compared to T1 group. Albumin showed a significant (P<0.05) in all of treated groups compared to C+ group. The reduction of nitro blue tetrazolium (NBT) by radical oxygen produce from neutrophils cell showed a significantly decreased (P<0.01) in C+ and C - groups compared to all treated groups. nanoparticle based treatment technologies could overcome threats of multi-drug resistance syndrome either as an alternative or as supplementary to oxytetracycline therapy and it reduces the use of antibiotics and the potential for environmental effects.