Polymorphismsof Filaggrin Gene as a New Marker to Detection of Gastric Cancer Patients in Iraq

Abstract

Gastric cancer is the most common cancer in the world specifically in Iraq, compared to other cancers, that affects patients of old ages of 30 years and more. This study deals with the verification of cancerous gene, through the filaggrin (FLG) gene, as well as to explain the relationship between filaggrin gene and other clinical indicators [Alp-fetoprotein (AFP), and Protein53 (P53)]in patients with gastric cancer, under study. Ninety patients with gastric cancer were selected from AL-Hussein Teaching Hospital of Kerbala and 100 healthy subjects represented as the control group, their ages were identical with the ages of patients.The clinical characteristics of patients were documented, which included age, smoking, family history, obesity, and chemotherapy drug. Statistical analysis of the results showed that 60% of patients were aged (51-65) year and 40% were between the ages of (30-50) year.Single Nucleotide Polymorphism (SNP) to Filaggrin (FLG) gene was measured in the blood of same patients and healthy group.

          Chi-square test was used to compare the differences in genotypeand allele frequencies of FLGrs2065955 in each group of genetic study to filaggrin (FLG) gene. Also student`s t-test was used to analyze the results. The statistical results were showed the cytosine-cytosine (CC) genotype was found in 47 of 90 (52.2%)  (P=0.003) in gastric cancer cases, and 13 of 100 (13%) in normal control, while the guanine-cytosine (GC) genotype was found in 23 of 90 (25.6%) (P=0.016) in gastric cancer cases, and 69 of 100 (69%) in normal control.Also the statistical results were documented a highly significant (P˂0.000) increase in the level of AFP. There also was a highly significant (P˂0.01) increase in P53 level, compared with the healthy group.