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The application of plants is well known to us and it is still espousing in the recent era. The plant kingdom is a treasure house for potential medicines thereby enhancing knowledge of medicinal plants in recent years. Cerium, an element in the sequence of lanthanides, shows oxidation state of Ce3+, Ce4+ and the ability to significantly change or quickly turn over its state. The methanolic extract from Morus alba leaves was prepared by using Soxhlet apparatus and the preliminary phytochemical investigationwas conducted and confirmed the presence of carbohydrates, steroids, amino acids, anthocyanins, phenols, tannins, alkaloids, flavonoids, terpenoids and glycosides. By observing the XRD pattern, the CdS NPs was confirmed by its characteristic peak. Furthermore,by using CdS NPs the antibacterial and antifungal activity against Salmonella typhi, Escherichia coli and fungal isolate Candida Albicans, A. Nigerhas been carried out. Maximum zone of inhibition is observed in Salmonella typhi and Candida albicans.